Effect of sodium phenylbutyrate on the apoptosis of human tongue squamous cancer cell line and expression of p21 and survivin genes
10.3760/cma.j.issn.1002-0098.2010.07.011
- VernacularTitle:苯丁酸钠对人舌鳞状细胞癌细胞凋亡影响机制的探讨
- Author:
Wei-Qiang CHEN
1
;
Feng-Lan FENG
;
Hong-Biao GU
;
De-Shun PAN
Author Information
1. 广东药学院
- Keywords:
Oncogene protein p21;
Tongue neoplasms;
Carcinoma,squamous cell
- From:
Chinese Journal of Stomatology
2010;45(7):416-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effects of sodium phenylbutyrate on the apoptosis of human tongue squamous cancer cell line and expression of p21 and survivin genes.Methods The inhibition effects of sodium phenylbutyrate on Tca8113 and human tongue squamous cell carcinoma (TCSSA) cell lines were detected by methyl thiazoly terazolium (MTT) and the apoptosis of the cancer cells after being induced by sodium phenylbutyrate examined by flow cytometry(FCM).The expression of p21 and survivin genes were observed with Western blotting and RT-PCR Results Compared with control group,the level of p21 mRNA and protein of Tca8113 cellline increased to 0.09±0.08 and increased 0.72±0.10,that of TCSSA cellline increased 1.34±0.12 and 1.56±0.09(P <0.05).Compared with control group,the level of surrive mRNA and protein of Tca8113 cellline decreased to 1.10±0.05 and 1.14±1.10,that of TCSSA cellline decreased to 0.12±0.08 and 0.94±0.09 (P < 0.05) .Sodium phenylbutyrate inhibited the cell proliferation ,promoted cell apoptosis and arrested the cells in G1/G0 phase.The amount of p21 mRNA and protein were increased,and the expression of survivin gene was decreased.Conclusions Sodium phenylbutyrate exhibited remarkable inhibitory effects on human tongue squamous cancer cell proliferation and induced cancer cell apoptosis.The mechanism may be due to up-regulation of p21 gene and downregulation of survivin gene.The mRNA level of p21 gene and survivin gene showed a strong correlation.
