Inhibitory effect of transforming growth factor-β1 on oral sqnamous cell carcinoma brain metastasis Tb cell line
10.3760/cma.j.issn.1002-0098.2010.07.013
- VernacularTitle:转化生长因子β1对口腔鳞状细胞癌脑转移细胞系Tb细胞生长抑制作用的研究
- Author:
Cheng-Min LIU
1
;
Cheng-Ren ZHANG
;
Xiu-Mei WANG
;
Xu-Guang XU
;
Song-Bin FU
Author Information
1. 哈尔滨医科大学附属第二医院
- Keywords:
Transforming growth factor batal;
Carcinoma,squamous cell;
Gene expression
- From:
Chinese Journal of Stomatology
2010;45(7):421-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of transforming growth factor (TGF) -β1 on oral squamous cell carcinoma (OSCC) Tb cell line.Methods Cell counting method was used to examine the inhibitory effect of TGF-β1 on Tb cell and flow cytometry(FCM) assay performed to measure the changes of cell cycle.Superarray was used to screen the changing expression of genes in TGF-β1/Smads signaling pathway.RT-PCR method was used to detect the results of Superarray.Results TGF-β1 showed significant inhibiting effect on OSCC Tb cell line.TGF-β1 blocked the cell cycle at G1 phase.The expression level of activin receptor-like kinase -1 (ACVRL-1),anti-mullerian hirmine (AMH),cyclim-dependent kinase inhibitor-2B (CDKN-2B) and transforming growth factor-beta-indnced factor(TGIF) was higer in the cells treated with TGF-β1 than in control,while TDGF-1 expression was down-regulated.ACVRL-1 and CDKN-2B gene expression was consistent with the results of Superarray.Conclusions TGF-β1 can inhibit the growth of OSCC Th cell line.The mechanism may be related to the regulation of cell cycle and the expression of ACVRL-I and CDKN-2B in TGF-β1-Smads signaling pathway.
