Study and preparation of a novel apatite-wollastonite bioactive glass-ceramic-calcium sulphate hemihydrate composite
10.3760/cma.j.issn.1002-0098.2010.07.017
- VernacularTitle:新型磷灰石-硅灰石生物活性玻璃陶瓷-半水硫酸钙复合材料的制备及研究
- Author:
Xin-Xin CHEN
1
;
Yun-Mao LIAO
;
Zhi-Min ZHU
Author Information
1. 成都军区总医院附属口腔医院
- Keywords:
Calcium sulfate;
Bone substitute;
Osteoblasts;
Apatite-wollastonite bioactive glass-ceramic
- From:
Chinese Journal of Stomatology
2010;45(7):439-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a novel apatite-wollastonite bioactive glass-ceramic-calcium sulphate hemihydrate(AW-BGC-CSH) composite,to study its biocompatibility,and to provide experimental support for its further clinical application.Methods Samples of AW-BGC-CSH composite were prepared with different AW-BGC granules-CSH ratios (50%,40%,30%,20%).Surface morphology,microstructure and mechanical features of the composite were measured.Osteoblasts were cultivated in vitro on the composite.Cell morphology,proliferation,and the alkaline phosphatase(ALP) activity of osteoblasts were examined to determine the biocompatibility of the composite.Results The composite showed a threedimensional pored structure with communicated micropores under scanning electron microscopy(SEM).The plasticity of the composite could be maintained within 3-5 min.Its top solidification temperature was 36.4 ℃and the maximum compressive strength was 9.3 Mpa.The osteoblasts adhered to the composite and grew well.At 1,3,5,7 d after cultivated,the microprotein contents of the composite were (251±12),(296±31),(580±13) and (571±15)mg/L,and the ALP activity of the composite were (4.50±0.68),(6.90±0.27),(12.05±0.28) and (11.86±0.63) U/mg.The results of the ALP activity and microprotein contents in the experiment group were significantly higher than those in the control group(P <0.05).Conclusions The prepared AW-BGC-CSH composite has a three-dimensional pored structure,favourable plasticity,mechanical property and good biocompatibility.
