Effects of RhoA siRNA on the proliferation, adhesion, migration and invasion of tongue squamous cell carcinoma Tca8113 cells in vitro.
- Author:
Jun ZHOU
1
;
Yong HE
;
Shao-Zhong DONG
;
Yuan LI
;
Yuan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; pathology; Cell Adhesion; Cell Cycle; Cell Line, Tumor; Cell Movement; Cell Proliferation; Humans; In Vitro Techniques; RNA, Small Interfering; Tongue Neoplasms; pathology; Transfection
- From: Chinese Journal of Stomatology 2010;45(9):520-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEto investigate the effect of RhoA on the metastasis of tongue squamous cell carcinoma Tca8113 cells in vitro.
METHODSa group of RhoA specific small interfering RNAs (siRNA) was constructed and confined by sequencing analysis. The siRNA of RhoA gene was transfected into human tongue squamous cell carcinoma Tca8113 cells line by Lipofectamine(TM) 2000. The protein transient expression of RhoA in the transfectants was examined 48 h after transfection. The cell line with stable expression of siRNA of RhoA was obtained by blasticidin screening and colony culture. Cell growth rate was determined with a cell counting. Millicell chambermodel and wound healing assay were used to examine the abilities of migration and invasion, respectively in vitro.
RESULTSRhoA was overexpressed in tongue squamous cell carcinoma Tca8113 cell line. Silencing of endogenous RhoA gene expression in Tca8113 cells resulted in inhibition of the proliferation, adhesion, chemotaxis and invasion of Tca8113 cells in vitro.
CONCLUSIONSRhoA siRNA inhibits the proliferation, adhesion, chemotaxis and invasion of oral squamous cell carcinoma Tca8113 cell lines. siRNA of RhoA is a potential factor controlling the proliferation and metastasis of Tca8113 cells. RhoA may play an important role in metastasis of oral squamous cell carcinoma.
