Comparative study on the ability of adhesion and invasion of different fimA genetypes of Porphyromonas gingivalis to oral epithelial cells.
- Author:
Li GAO
1
;
Ya-fei WU
;
Di MIAO
;
Lei ZHAO
;
Shu MENG
;
Jie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Adhesion; Chronic Periodontitis; microbiology; Epithelial Cells; microbiology; Fimbriae Proteins; genetics; physiology; Genetic Variation; Genotype; Humans; KB Cells; microbiology; ultrastructure; Microscopy, Electron, Scanning; Porphyromonas gingivalis; genetics; isolation & purification; physiology
- From: Chinese Journal of Stomatology 2010;45(6):342-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the ability of adhesion and invasion to epithelial cells by Porphyromonas gingivalis (Pg) strains with different fimA separated from Chinese.
METHODSCultured method and antibiotic protection method were used to determine the adhesive and invasive ability of Pg with different fimA genetypes. The adhesion was observed by scanning electron microscope.
RESULTSAll the strains adhered and invaded to KB cells, and the adhesion rate ranged from 0.523% to 37.125% and invasive rate from 0.017% to 3.750%.The adhesive and invasive ability among different fimA genotypes showed no significant difference (P > 0.05).
CONCLUSIONSThere is no significant correlation between fimA genotype and ability in adhesion and invasion to KB cells.
