Analysis of outer membrane proteins in various strains of Porphyromonas gingivalis by surface enhanced laser desorption/ionization time-of-flight mass spectrometry
10.3760/cma.j.issn.1002-0098.2010.10.011
- VernacularTitle:应用表面增强激光解吸电离飞行时间质谱技术筛选牙龈卟啉单胞菌共同外膜蛋白
- Author:
Ang LI
1
;
Jun-Yi SUN
;
Yuan-Yuan SUN
;
Jian-Feng SHI
;
Guo-Zhou RAO
;
Jian-Zhong GOU
Author Information
1. 西安交通大学医学院附属口腔医院
- Keywords:
Porphyromonas gingivalis;
Bacterial outer membrane proteins;
Surface enhanced laser desorption/ionization time-of-flight mass spectrometry
- From:
Chinese Journal of Stomatology
2010;45(10):614-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the common outer membrane proteins (OMP)from Porphyromonas gingivalis (Pg)by surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and to provide antigens for the subsequent experiments in screening vaccine for periodontitis therapy. Methods Four strains of Pg were cultured under anaerobic conditions. The common OMP was extracted through ultracentrifugation and SELDI-TOF-MS was employed to detect the expressions of proteomes by chip H50. The data was analyzed by Biomarker Wizard. ResultsFour kinds of strains of OMP fingerprint spectrum were obtained. Seventy-one proteins of PgATCC33277, 74 proteins of PgW83, 76 proteins of PgW301 and 72 proteins of Pg381 were captured by chip H50. Thirteen common proteins were identified according to fingerprint spectrum. There was only 1 of the 13 common proteins identified in NCBI protein bank. ConclusionsSELDI-TOF-MS has good reproducibility and high sensibility and can be used to identify the common OMP of Pg. The 13 proteins have a potential value in the screening vaccine candidate antigen sites for periodontitis.
