Effects of cofilin phosphorylation on the actin cytoskeleton reorganization induced by shear stress
10.3760/cma.j.issn.1002-0098.2010.12.015
- VernacularTitle:丝切蛋白在成骨细胞细胞骨架改建中的作用探讨
- Author:
Yan-Hui LIU
1
;
You-Rui LI
;
Min-Feng SHAO
;
Xiao-Juan ZHANG
;
Qiang FU
Author Information
1. 广州中医药大学附属第一医院
- Keywords:
Osteoblasts;
Cytoskeleton;
Actin depolymerizing factors;
Fluid shear stress
- From:
Chinese Journal of Stomatology
2010;45(12):763-766
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of cofilin on the actin eytoskeleton reorganization in osteoblasts induced by fluid shear stress. Methods Fluid shear stress ( 1.2 Pa) was applied to osteoblasts for 0(control group), 15, 30, 45, 60, 120 min in vitro. Cells were stained with fluorescein isothiocyanate (FITC)-phalloidin for fiber-actin, and confocal laser scanning microscope( CLSM ) was used to observe the fluorescence of fiber-actin. Western blotting was used to detect the expression of the cofilin and the phosphocofilin. Results Actin filaments became organized into stress fibers that were thicker and more abundant than those in non-flowed cells. The fluorescence intensity ( 38. 00 ± 6. 88 ) of fiber-actin after 120 min (42.93 ±6.41) loading it was 2.8 times as much as that in control group(15.41 ±3.60, P ＜0.05).Additionally, the level of phospho-cofilin protein was dramatically elevated after loading Fluid shear stress induced an initial decrease of cofilin at 60 min. However, at 120 min cofilin(0. 254 ±0. 026) increased to 1.5 times as much as that at 60 min (0. 162 ± 0. 004 ). Conclusions The results indicate that cofilin phosphorylation mediates fiber-actin reorganization in the osteoblasts induc ed by fluid shear stress.
