Establishment of chronic periodontitis and chronic obstructive pulmonary disease model in SD rat
10.3760/cma.j.issn.1002-0098.2010.11.009
- VernacularTitle:牙周炎和慢性阻塞性肺疾病动物模型的建立及其相关性分析
- Author:
Dong-Xue ZHANG
1
;
Mei LIN
;
Bao-Sen PANG
;
Jing ZHANG
;
Zuo-Min WANG
Author Information
1. 首都医科大学附属北京朝阳医院
- Keywords:
Periodontitis;
Pulmonary disease;
chronic obstructive;
Models,animal
- From:
Chinese Journal of Stomatology
2010;45(11):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a model of chronic periodontitis(CP) accompanied with chronic obstructive pulmonary disease (COPD) in SD rats and investigate the relationship between chronic periodontitis and COPD. Methods Equal gender SD rats were randomly divided into 4 groups, A: control group, B: CP group, C: COPD group, D: COPD with CP group (n = 10, respectively). Each group was subjected to its predesigned intervention to establish a specific disease model. After 10 weeks, animals were sacrificed. The level of alveolar bone loss, lung function measurement, and the histopathological changes of periodontal and lung tissues were examined. The serum tumor necrosis factor (TNF)-α level was detected by enzymelinked immunoabsorbent assay kits. Results Bleeding index (BI) levels of group A and C were (0.25±0.04) and (1.30 ±0.25),respectively. Attachment loss was (0.43 ±0.02) and (0.51 ±0. 02) mm. BI levels in group B and D were significantly higher than those in group A and C. Forced expiratory volume in 0. 2 second to forced vital capital ratio(FEV0.2/FVC) values in group B, C and D were significantly lower than that in group A. Pulmonary function were worse in group D than that in group C(P＜0. 05). The levels of serum TNF-α, a sensitive indicator of both diseases, were increased in all test groups compared with the control, and increased most in group D. Conclusions The chronic periodontitis and chronic obstructive pulmonary disease model was established in SD rat. The chronic periodontitis may be a risk factor for promoting and inducing COPD.
