Effect of basic fibroblast growth factor on the gene expression of epidermal growth factor receptor by periodontal ligament cells in culture.
- Author:
Ying CHEN
1
;
Chong-tao LIN
;
Ying GAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cells, Cultured; Fibroblast Growth Factor 2; Humans; Periodontal Ligament; RNA, Messenger; Receptor, Epidermal Growth Factor; Regeneration
- From: West China Journal of Stomatology 2009;27(3):340-343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of basic fibroblast growth factor (bFGF) on the gene expression of epidermal growth factor receptor (EGFR) by periodontal ligament cells (PDLC) in culture and discuss the effect of bFGF in cell differentiation and periodontal regeneration.
METHODSHuman PDLC were cultured in vitro and cell clone was obtained by the method of limiting dilution. PDLC were stimulated by bFGF, and then gene expression of EGFR was assessed by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSThe mRNA expression of EGFR was promoted by bFGF and the effect was dose-dependent.
CONCLUSIONThe promotion effect of the mRNA expression of EGFR in PDLC may be one of important factors which participate in the healing process of periodontitis and provide partly theoretical basis of cell differentiation and periodontal regeneration.
