Heterotopic complex membrane induces bone formation.
- Author:
Chong-tao LIN
1
;
Hong-yan LI
;
Jia-qing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Morphogenetic Protein 2; Collagen; Glycolates; Lactic Acid; Male; Mice; Osteogenesis; Polyesters; Polyglycolic Acid; Polymers; Recombinant Proteins; Transforming Growth Factor beta
- From: West China Journal of Stomatology 2009;27(4):357-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen (Co), polylactic-co-glycolic acid (PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane.
METHODS48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination.
RESULTSWounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X-ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane.
CONCLUSIONCo/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.