The effect of Nifedipine and mechanical strain on matrix metalloproteinase expression in human periodontal ligament fibroblast.

Hui NI; Miao-miao Zhang; Jia-yin Dai; Miao Sun

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The effect of Nifedipine and mechanical strain on matrix metalloproteinase expression in human periodontal ligament fibroblast.

Author: Hui NI ¹ ; Miao-miao ZHANG ; Jia-yin DAI ; Miao SUN
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1. Dept. of Orthodontics, School of Stomatology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Publication Type:Journal Article
MeSH: Fibroblasts; Humans; Matrix Metalloproteinases; Nifedipine; Periodontal Ligament; RNA, Messenger
From: West China Journal of Stomatology 2009;27(4):366-369
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effect of Nifedipine on matrix metalloproteinase (MMP)-10 and MMP-13 expression in human periodontal ligament fibroblast (HPDLF) induced by mechanical strain in vitro.
METHODSHPDLF was divided into four groups at random by mechanical strain of elongation 0%, 8%, 12% and 16%. Each group was divided into four subgroups again by the concentration of Nifedipine 0, 10, 30, 50 microm. The cells were imposed to state mechanical strain 12 h after incubated with Nifedipine 1 h. And then immunohistochemical staining was used to investigate the expressions of MMP-10 and MMP-13 in intracytoplasm.
RESULTSIn 0% group, there was no significant difference of MMP-10 and MMP-13 expression in all subgroups (P>0.05). The expressions of MMP-10 and MMP-13 were high in group 8%, 12% and 16% without Nifedipine, and increasing significantly with elongation (P<0.001). The expressions of MMP-10 and MMP-13 decreased with dose increasing (P<0.001) in HPDLF after imposed to Nifidepine.
CONCLUSIONThe expressions of MMP-10 and MMP-13 induced by mechanical strain were inhibited by Nifedipine, which suggested calcium ions channels participate in expressions of MMP-10 and MMP-13 induced by loading.