Effects of tumor necrosis factor-alpha on the growth of rat osteoblasts.
- Author:
Li-wei XUE
1
;
Jun ZHANG
;
Xu-xia WANG
;
Tao BU
;
Mei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Animals; Cell Differentiation; Cell Proliferation; Osteoblasts; Rats; Tumor Necrosis Factor-alpha
- From: West China Journal of Stomatology 2009;27(4):378-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of tumor necrosis factor alpha (TNF-alpha) on the growth of rat osteoblasts. To find out the mechanisms that TNF-alpha regulates the growth of osteoblasts.
METHODSTo assay osteoblasts proliferation by MTT. To assay alkaline phosphatase (ALP) activity of osteoblasts by PP-nitrophenyl phosphate (PNPP).
RESULTSThe osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower levels were observed in above 50 ng/mL groups (P<0.05).
CONCLUSIONTNF-alpha restrained osteoblasts proliferation and differentiation, and the effects were more significant in above 50 ng/mL groups.
