Nicotine affects biological properties of the human osteosarcoma cell line MG63.
- Author:
Bing BAI
1
;
Li-fang ZHONG
;
Jing-tao ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Bone Neoplasms; Cell Line; Cell Proliferation; Collagen Type I; Humans; Nicotine; Osteoblasts; Osteocalcin; Osteogenesis; Osteosarcoma
- From: West China Journal of Stomatology 2009;27(5):483-486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of nicotine on cell proliferation.
METHODSThe cells were cultured with DMEM medium containing 10% fetal bovine serum with 0, 1 x 10(-4) mol x L(-1), and 1 x 10(-3) mol x L(-1) nicotine for up to 3, 5, 7, 10, 14 days. The cell proliferation was evaluated by MTT. The alkaline phosphatase (ALP) activity was estimated by PNPP. The expression of collagen type I (COL1) and osteocalcin (OCN) were estimated by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSNicotine suppressed the cell proliferation. ALP activity increased to peak on 10 days in control and 1 x 10(-4) mol x L(-1) nicotine. COL1 expression increased to peak on 10 days in control and 1 x 10(-4) mol x L(-1) nicotine, but there was decreased to the minimum on 10 days and increased on 14 days in 1 x 10(-3) mol x L(-1) nicotine. OCN expression increased to peak on 10 days in control, and increased in 1 x 10(-4) mol x L(-1) nicontine from 3 days to 14 days, but there wasn't significant change in 1 x 10(-3) mol x L(-1) nicontine.
CONCLUSIONNicotine suppresses osteogenesis through a decrease in ALP and COL1 production by osteoblasts.
