Relationship between Pulmonary Surfactant Protein and Lipid Peroxidation in Lung Injury due to Paraquat Intoxication in Rats.
10.3904/kjim.2007.22.2.67
- Author:
Hyo Wook GIL
1
;
Mi Hae OH
;
Kee Min WOO
;
Eun Young LEE
;
Myung Ho OH
;
Sae Yong HONG
Author Information
1. Department of Internal Medicine, Soonchunhyang University Medical College, Cheonan Hospital, Cheonan, Korea. eylee@sch.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Paraquat;
Lipid peroxidation;
Surfactant protein A;
Surfactant protein D
- MeSH:
Animals;
Free Radicals/*toxicity;
Herbicides/*toxicity;
*Lipid Peroxidation;
Lung/*drug effects;
Male;
Paraquat/*toxicity;
Pulmonary Surfactant-Associated Proteins/*analysis;
Rats;
Rats, Sprague-Dawley;
Reactive Oxygen Species/toxicity;
Respiratory Distress Syndrome, Adult/*chemically induced
- From:The Korean Journal of Internal Medicine
2007;22(2):67-72
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Pulmonary damage resulting from lipid peroxidation is a principal effect of paraquat intoxication. The host-defense functions of surfactant are known to be mediated by the surfactant proteins A and D (SP-A and SP-D, respectively). The primary objective of this study was to evaluate the variations over time in levels of surfactant protein and lipid peroxidation (LPO) in lung tissue following free-radical-induced injury. METHODS: 42 adult, male, Sprague-Dawley rats were administered intraperitoneal injections of paraquat (35 mg/kg body weight). SP-A and SP-D levels were determined via Western blot. LPO in the left lung homogenate was measured via analyses of the levels of thiobarbituric acid-reactive substances. RESULTS: LPO levels peaked at 6 hours, with no associated histological changes. SP-D levels increased until hour 12 and declined until hour 48; SP-D levels subsequently began to increase again, peaking at hour 72. SP-A levels peaked at hour 6, declining thereafter. CONCLUSIONS: We suggest that in the early phase of paraquat injury, SP-D levels reflect alveolar damage and that de novo synthesis of SP-D takes 72 hours. Levels of SP-A, on the other hand, reflect abnormalities in the surfactant system in the late stage of paraquat intoxication. Surfactant proteins may play a role in protecting the lungs from reactive oxygen injury. A time-dependent variation has been observed in the levels of surfactant proteins A and D following paraquat injury, and it has been suggested that these proteins play a role in the protection of lung tissue against ROS-induced injuries.
