Effects of Drynaria fortunei naringin on proliferation, alkaline phosphatase activity of human periodontal ligament cells.
- Author:
Jun-qiang JIANG
1
;
Yi DING
;
Xiao-yu LI
;
Wei CAI
;
Zhong-chao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Cell Proliferation; Cells, Cultured; Drugs, Chinese Herbal; Flavanones; Humans; Osteoblasts; Periodontal Ligament; Polypodiaceae
- From: West China Journal of Stomatology 2009;27(5):538-541
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the biological effects of Drynaria fortunei naringin on the proliferation and alkaline phosphatase (ALP) activity of human periodontal ligament cells (hPDLC).
METHODShPDLC were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to observe the proliferation of hPDLC with the effects of different concentrations of Drynaria fortunei naringin at difference times. The method recommended by International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) was adopted to investigate the effect of Drynaria fortune naringin on the alkaline phosphatase (ALP) activity of hPDLC. At the same time, we used bright blue method to detect the contents of protein in each sample. Then ALP activity in per milligram protein was accounted.
RESULTSSignificant proliferative promotion to hPDLC by Drynaria fortunei naringin could be observed at the dose of 10, 1, 0.1 mg x L(-1). Significant ALP activity of hPDLC promotion of Drynaria fortune naringin could be observed at the dose of 100, 10, 1, 0.1 mg x L(-1) and the dose of 1 mg x L(-1) Drynaria fortune naringin had greatest promotion on ALP activity of hPDLC.
CONCLUSIONDrynaria fortune naringin might significantly promote the proliferation and increase the ALP activity of hPDLC.
