Culture and identify the human umbilical vein endothelial cells and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains in the cells.
- Author:
Shi-qing WU
1
;
Jun-fa ZHENG
;
Shu-guang ZENG
;
Shao-peng CHEN
;
Guo-chu XUE
;
Ji
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; EGF Family of Proteins; Endothelial Cells; Human Umbilical Vein Endothelial Cells; Humans; Immunoglobulins; TYK2 Kinase; Umbilical Veins
- From: West China Journal of Stomatology 2009;27(6):653-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cultural method and identification of human umbilical vein endothelial cells (HUVECs), and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains(Tie-2) in HUVECs.
METHODSHUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by VIII monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and SABC immunocytochemistry.
RESULTSHUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85%.
CONCLUSIONHighly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.
