False human immunodeficiency virus test results associated with rheumatoid factors in rheumatoid arthritis.
- Author:
Yun-Chun LI
1
;
Fan YANG
1
;
Xiao-Yun JI
1
;
Zhong-Jun FANG
1
;
Jun LIU
1
;
Yue WANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Arthritis, Rheumatoid; physiopathology; Enzyme-Linked Immunosorbent Assay; False Positive Reactions; HIV Antibodies; blood; HIV Antigens; blood; HIV Infections; diagnosis; Humans; Middle Aged; Rheumatoid Factor; physiology
- From: Chinese Medical Sciences Journal 2014;29(2):103-106
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate if immunological factors associated with rheumatoid arthritis (RA) affect the result of human immunodeficiency virus (HIV) screening by electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA).
METHODS100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors (RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA.
RESULTSThe HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method (P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA (P<0.01).
CONCLUSIONSImmunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.
