Effects of H2O2 and ascorbic acid on TIMP-2, Type 1 collagen, and PDLs22 levels in human periodontal ligament fibroblasts.
10.5051/jkape.2007.37.4.655
- Author:
Yong Sun CHOI
1
;
So Young KIM
;
Seong Mi CHOI
;
Hyun Seon JANG
;
Byung Ock KIM
Author Information
1. Department of Periodontology, College of Dentistry, Chosun University, Korea. bobkim@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
H2O2;
ascorbic acid;
human periodontal ligament fibroblasts
- MeSH:
Antioxidants;
Ascorbic Acid*;
Collagen Type I*;
Fibroblasts*;
Fibronectins;
Gene Expression;
Humans*;
Matrix Metalloproteinase 1;
Periodontal Ligament*;
Periodontium;
Reactive Oxygen Species;
Regeneration;
RNA, Messenger;
Tissue Inhibitor of Metalloproteinase-1;
Tissue Inhibitor of Metalloproteinase-2*
- From:The Journal of the Korean Academy of Periodontology
2007;37(4):655-669
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases. And vitamin C has shown a protective effect for the tissues. The aim of this study was to evaluate the effects of H2O2 and ascorbic acid on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase (TIMP: TIMP-1, TIMP-2), Type 1 collagen, fibronectin, and PDLs22 level in human periodontal ligament fibroblasts (hPDLF) via reverse transcription-polymerase chain reaction (RT-PCR). hPDLF was obtained from a healthy periodontium and cultured in Dulbecco's modified Eagles's medium plus 10% fetal bone serum. The concentration of ascorbic acid in hPDLF was 50 microgram/ml, and that of H2O2 in hPDLF was 0.03% and 0.00003%. Ascorbic acid only, H2O2 only and mixture of ascorbic acid and H2O2 were applied with hPDLF for 1-, 3-, and 30-min., respectively. The gene expression of MMP-1-, TIMP-1-, TIMP-2-, Type 1 collagen-, fibronectin-, and PDLs22-mRNA in hPDLF was analysed via RT-PCR. The results were as follows; 1. hPDLF in response to 30-min. incubation with 0.03% H2O2 did not show any gene expression. 2. In all the experimental groups, the gene expression of fibronectin mRNA showed the decreased tendency compared to control. 3. In all the experimental groups, the gene expression of TIMP-1 mRNA showed the tendency similar to control. 4. hPDLF in response to 30-min. incubation with 0.03% H2O2 and ascorbic acid increased mRNA induction for MMP-1. 5. In all the experimental groups, hPDLF increased mRNA induction for PDLs22, collagen type I, and TIMP-2 compared to control. Within the limited experiments, H2O2 and ascorbic acid increased mRNA induction for PDLs22, collagen type I, and TIMP-2 in hPDLF. More research will be needed in order to confirm the relative importance of the different roles of ROS and antioxidants in hPDLF from a periodontal regeneration or repair standpoint.
