The Gingival Tissue Levels of Bone Resorptive Mediators in Human Chronic Periodontitis with Type 2 Diabetes Mellitus.
10.5051/jkape.2007.37.4.743
- Author:
Mi Jeong KIM
1
;
Sang Ho RYU
;
Jin Woo PARK
;
Jo Young SUH
;
Jae Mok LEE
Author Information
1. Department of Periodontology, School of Dentistry, Kyungpook National University, Korea. leejm@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Chronic periodontitis;
diabetes mellitus;
bone resorption factor
- MeSH:
Adult;
Bone Resorption;
Chronic Periodontitis*;
Diabetes Mellitus;
Diabetes Mellitus, Type 2*;
Dinoprostone;
Gingiva;
Hemorrhage;
Humans*;
Inflammation;
Interleukin-6;
Periodontal Pocket;
Tooth Extraction
- From:The Journal of the Korean Academy of Periodontology
2007;37(4):743-753
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purposes of this study were to compare and quantify the expression of MMP-3, PGE2 and IL-6 in the gingival tissues of patients with type2 diabetes mellitus and healthy adults with chronic periodontitis. MATERIAL AND METHODS: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1(n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2(n=8) is inflamed gingiva from patients with chronic periodontitis. Group 3(n=8) is inflamed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Westernblotting. The quantification of MMP-3, PGE2 and IL-6 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. RESULTS: 1. The expression levels of MMP-3 were shown highest in group 3 compared to group 1 and 2, and It showed increasing tendency in group 2 and 3. 2. The expressions of PGE2 and IL-6 were shown increasing tendency in group 2 and 3, and It was highest in group 3. 3. As expressions of MMP-3 were increased, PGE2 and IL-6 expressions showed increasing tendency in group 3 than group1 and 2, although there were no proportional relationship. CONCLUSION: This study demonstrated that the expression levels of MMP-3, PGE2 and IL-6 will be inflammatory markers of periodonta linflamed tissue and DM. It can be assumed that MMP-3 affect to expressions of PGE2 and IL-6 in progression of periodontal inflammation with alveolar bone resorption to type 2 DM.
