Multilocus sequence typing of methicillin-resistant Staphylococcus aureus.
- Author:
Xiao-mei YAN
1
;
Yi-xin GU
;
Li-hua HE
;
Hui-fang ZHANG
;
Jian-zhong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Typing Techniques; Base Sequence; DNA, Bacterial; genetics; Genes, Bacterial; Methicillin-Resistant Staphylococcus aureus; classification; drug effects; genetics
- From: Chinese Journal of Preventive Medicine 2009;43(2):137-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA.
METHODSSequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the allelic profiles were gained from internet database.
RESULTSAll 12 MRSA strains in 2000 were sequence type (ST) 239 and 10 MRSA strains in 2005 were ST239, while 7 MRSA strains in 2005 were new types, ST5 (41.18%, 7/17). ST6 and ST630 were allelic profiles of 2 MSSA strains. ST239 was the most prevalent allelic profile (75.86%, 22/29), while ST5 was the second prevalent allelic profile (24.14%, 7/29) among all isolates.
CONCLUSIONST239 and ST5 are the most prevalent MRSA clones in this research. MRSA strains have different allelic profile from MSSA strains. MLST might provide an unambiguous method for assigning MRSA and MSSA isolates to known clones or assigning them as novel clones via the internet. Further studies need to be taken by increasing strains.
