A new member of Brevidensovirus, 0507JS11 virus isolated from Culex mosquitoes collected in Xinjiang.
- Author:
Xin-jun LÜ
1
;
You-gang ZHAI
;
Xiao-hong SUN
;
Shi-hong FU
;
Huan-qin WANG
;
Su-xiang TONG
;
Song ZHANG
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Culex; virology; DNA, Viral; genetics; Densovirinae; classification; genetics; isolation & purification; Genome, Viral; Sequence Analysis, DNA
- From: Chinese Journal of Preventive Medicine 2009;43(5):385-389
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo probe the primary characteristic of 0507JS11 virus isolated from Culex sp. and determine the classification of 0507JS11 virus in taxonomy.
METHODS0507JS11 virus was cultured in Aedes albopictus C6/36 cells and cytopathic effects (CPEs) were recorded. Electro-microscopic morphology of 0507JS11 virus was observed. Total DNA extract of 0507JS11 virus was detected by 1% Agarose Gel Electrophoresis. Complete genomic sequence of 0507JS11 virus was sequenced and then made phylogenetic analysis.
RESULTS0507JS11 virus could cause CPEs in Aedes albopictus C6/36 cells. Viral particles have no envelope and appear icosahedron symmetry with diameter of 20 nm. The genome of 0507JS11 virus was positive single strand DNA (ssDNA) with full length of 3977 nt. However, a DNA band about 4 kbp was observed in the electrophoresis of total DNA extract of 0507JS11 virus. The coding region of the genome included three ORFs, ORF1 and ORF2 code NSP1 and NSP2, ORF3 codes VP. Phylogenetic analysis of the complete genomic sequence of 0507JS11 virus indicated an independent linear in Brevidensovirus.
CONCLUSION0507JS11 virus is a new member in Brevidensovirus.
