Isolation and primary identification of viruses in mosquitoes in the south of Xinjiang.
- Author:
Xin-jun LÜ
1
;
Zhi LÜ
;
Xiao-hong SUN
;
Shi-hong FU
;
Huan-qin WANG
;
Su-xiang TONG
;
Song ZHANG
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bluetongue virus; classification; genetics; isolation & purification; Cercopithecus aethiops; China; Culicidae; virology; Dengue Virus; classification; genetics; isolation & purification; Genome, Viral; Insect Viruses; classification; genetics; isolation & purification; RNA, Double-Stranded; genetics; RNA, Viral; genetics; Reassortant Viruses; genetics; Sequence Analysis, DNA; Vero Cells
- From: Chinese Journal of Preventive Medicine 2009;43(5):395-398
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate viruses from mosquitoes in the south of Xinjiang and identify these viruses primarily.
METHODSA total of 13 491 mosquitoes were collected in the south of Xinjiang from Jul to Aug, 2005. These mosquitoes were divided into 130 groups and grinded respectively. The supernates were inoculated in C6/36 and Vero cells. Viruses isolated were detected, the genomic nucleic types by electrophoresis of viral genomes and the morphologies observed under electronmicroscope.
RESULTSAll 42 viruses were isolated, which caused CPEs on C6/36 but not on Vero cells. 27 viruses showed similar genomic profiles with 12 dsRNA segments. 1 virus displayed genomic profile with 10 dsRNA segments. 5 viruses took on similar genomic profiles with about 4 kbp DNA band. 9 viruses did not get any taxonomy information. Electromicroscopic pictures of these viruses revealed that above four types of viruses had distinguished morphologies indicating different virus species.
CONCLUSIONThere should be several virus species in the mosquitoes in the south of Xinjiang. dsRNA virus with 12 genomic segments should play analysis a predominant role in the south of Xinjiang.
