Preparation of gene chip for detecting different expression genes involved in aflatoxin biosynthesis.
- Author:
Na HU
1
;
Yang XU
;
Zhui TU
Author Information
- Publication Type:Journal Article
- MeSH: Aflatoxins; biosynthesis; Gene Expression Profiling; Oligonucleotide Array Sequence Analysis; methods
- From: Chinese Journal of Preventive Medicine 2009;43(5):423-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the methodology of gene chip to analyse genes involved in aflatoxin biosynthesis.
METHODSIn comparing reversed transcriptional PCR with gene chip, the gene chip was used to detect genes involved in aflatoxin biosynthesis.
RESULTSAfter arrayed the slide was incubated in water for 2 hours, exposed to a 650 mJ/cm2 of ultraviolet irradiation in the strata-linker for 30 s, roasted under 80 degrees C for 2 hours in oven, pre-hybridized for 45 minutes and dealt with other procedures. Finally, the slide was hybridized with fluor-derivatized sample at 42 degrees C for 16 hours.
CONCLUSIONWith the reasonable probe design and applicable protocol, the gene chip was prepared effectively for research on genes involved in aflatoxin biosynthesis.
