Virological and serological surveys for H9N2 subtype of influenza A virus in chickens and men in Shenzhen city.
- Author:
Xiaowen CHENG
1
;
Jianjun LIU
;
Jianfan HE
;
Fuxiang SHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; Chickens; China; epidemiology; Hemagglutination Inhibition Tests; Humans; Influenza A Virus, H9N2 Subtype; Influenza A virus; classification; immunology; isolation & purification; Influenza, Human; epidemiology; virology; Seroepidemiologic Studies
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(4):319-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand the distribution of influenza A H9N2 virus in chickens and men in Shenzhen area.
METHODSVirus isolation was performed in embryonated hen s eggs with routine method. The antibody to H9N2 virus was detected with micro-hemagglutination inhibition (HI) test, then the results were checked by using the neutralization assay in MDCK cells.
RESULTSTotally 27 strains of influenza A H9N2 virus were isolated from chickens in farm markets in Shenzhen, whereas no H9N2 virus was isolated from men. Approximately 26% of human sera with the HI titers > or =20 to H9N2 virus were detected. However only 7% of chicken sera with the HI titers > or =20 to H9N2 virus were detected. Meanwhile the HI titer and (MGT) of antibody to H9N2 virus in human sera increased with age. It was also found that there was a close relationship between HI antibody titer to H9N2 virus in human sera and occupation.
CONCLUSIONSThe distribution of influenza A H9N2 virus in chicken and men in Shenzhen was rather wide. The human H9N2 virus infection probably derived from chicken H9N2 virus.
