Antiviral effect of recombinant antibody to influenza virus HA antigen.
- Author:
Guibin YANG
1
;
Yan WANG
;
Xiaodong ZHAO
;
Feng HAN
;
Shuhua WU
;
Yunde HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; Antibodies, Viral; immunology; Antibody Specificity; Antigens, Viral; immunology; Cell Line; Influenza A virus; immunology; Male; Mice; Mice, Inbred BALB C; Neutralization Tests; Orthomyxoviridae Infections; prevention & control
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(4):337-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEStudy on the antiviral effect in vivo and in vitro of recombinant antibodies with neutralization activity to influenza virus HA antigen, IgG-IV-2 and IgG-IV-6 obtained from expression in Baculo/insert system.
METHODSViral titer of influenza virus in MDCK was compared before and after antibody application, and measured viral titer in mouse lung before and after antibodies applied on mucous membrane.
RESULTSIgG-IV-2 and IgG-IV-6 could reduce the titer from 4.5 log10 TCID50 to half is by 50% at doses 0.8 microg and 0.5 microg respectively. When recombinant antibodies were used on mucous membrane, the IgG-IV-2 and IgG-IV-6 reduced the titer by half at doses 0.25 mg/kg weight and 0.1 mg/kg weight respectively. The dose was 0.08 mg/kg weight when the two antibodies were jointly used.
CONCLUSIONSThe recombinant antibodies have antiviral effect in vivo and in vitro, they can neutralize viral virulence.
