Expression of full-length hepatitis E virus structural gene using baculovirus expression system.
- Author:
Mingcheng ZHANG
1
;
Yoo YI
;
Chongbai LIU
;
Shengli BI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Baculoviridae; genetics; Cells, Cultured; Gene Expression; Genes; genetics; Genetic Vectors; Hepatitis E virus; genetics; Recombinant Proteins; biosynthesis; genetics; Viral Structural Proteins; biosynthesis; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(4):354-356
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain recombinant baculovirus containing full-length structural gene of HEV and to express HEV structural protein.
METHODSFull-length structural gene of HEV (5147-7126 nt) was inserted into baculovirus expression vector pAcUW51. The recombinant plasmid and Baculo Gold DNA were co-transfected into insect cell line Sf9. Single plaque was picked and amplified and expression of HEV structural protein was tested by SDS-PAGE, Western blot and immunofluorescence methods.
RESULTSSDS-PAGE analysis showed HEV structural protein was highly expressed; Western blot and immunofluorescence assay showed that the expression product could specifically react with HEV positive sera, confirming the protein possessing HEV specific antigenicity.
CONCLUSIONSHEV structural protein was successfully expressed using baculovirus expression system.
