Effect of B7-2 expression plasmid on the specific immune response induced by HBV DNA vaccine.
- Author:
Yanjun WANG
1
;
Qinghan JIN
;
Changwen SONG
;
Bin SUN
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; Animals; Antigens, CD; immunology; B7-2 Antigen; Hepatitis B Antibodies; biosynthesis; Hepatitis B Vaccines; immunology; Hypersensitivity, Delayed; immunology; Male; Membrane Glycoproteins; immunology; Mice; Mice, Inbred C57BL; Plasmids; T-Lymphocytes, Cytotoxic; immunology; Vaccines, DNA; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2002;16(4):370-372
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether B7-2 could improve hepatitis B virus (HBV)-specific immunity induced by inoculation of a plasmid encoding HBV preS2+S HBV DNA vaccine.
METHODSB7-2 expression plasmids were inoculated with the DNA vaccine in the gastrocemius muscles. Then the cytolytic T Lymphocyte activity (CTL), the delayed-type hypersensitivity (DTH) response and the titers of anti-HBs were analysed.
RESULTSThe DTH response and CTL activity were significantly enhanced when B7-2 expression plasmids were coinoculated with the DNA vaccine (P<0.01), but the titers of anti-HBs were unaffected (P>0.05).
CONCLUSIONSThe results suggest that B7-2 expression plasmid could improve HBV specific cell-mediated immunity induced by HBV DNA vaccine, and the gene-based coinoculation strategy using HBV viral antigen and B7-2 costimmulating could be a powerful means of combating HBV infection.
