Expressions of SE-1, CD31 and CD105 in the vascular endothelial cells and serum of rat with hepatocellular carcinoma.
- Author:
Jing-yu WANG
1
;
Xiao-yuan XU
;
Jing-hui JIA
;
Chi-hong WU
;
Ruo-wen GE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD; blood; Carcinoma, Hepatocellular; blood supply; chemistry; Endothelial Cells; chemistry; immunology; Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Liver Neoplasms, Experimental; blood supply; chemistry; Male; Neovascularization, Pathologic; blood; Platelet Endothelial Cell Adhesion Molecule-1; blood; Rats; Rats, Inbred BUF
- From: Chinese Medical Journal 2010;123(6):730-733
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDHepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. In order to investigate the molecular biologic mechanism of HCC's development, we studied the expressions of SE-1, CD105 and CD31 in tumor endothelial cells (TECs) of HCC and in the serum of rats.
METHODSWe analyzed the expressions of SE-1, CD31 and CD105 in rat HCC tumor tissues using immunohistochemistry (IHC). Twenty HCC bearing rats and eighteen normal rats were examined for the expressions of SE-1, CD31 and CD105 antigens in serum by enzyme-linked immunosorbent assay (ELISA).
RESULTSSE-1, CD31 and CD105 antigens were detected both in HCC tissue and in normal liver tissue with higher expressions of CD31 and CD105 in HCC while the SE-1 antigen expression was higher in normal liver. Similarly, serum CD31 and CD105 in rats with HCC were significantly increased compared with normal rats (t = 2.8628, P = 0.0086; t = 4.4922, P < 0.0001, respectively). In contrast, SE-1 antigen in HCC rat serum was significantly decreased compared with normal rats (t = 3.4983, P = 0.0011).
CONCLUSIONSE-1, CD31 and CD105 are closely related with liver tumor angiogenesis, which is similar to their performances in terms of their expressions in the serum.
