Effect of beta3-adrenergic agonists on alveolar fluid clearance in hypoxic rat lungs.
- Author:
Nai-jing LI
1
;
Wei LI
;
Ping HE
;
Xiu GU
;
Sheng-qi LI
Author Information
- Publication Type:Journal Article
- MeSH: Adrenergic beta-Agonists; therapeutic use; Animals; Body Fluids; drug effects; metabolism; Ethanolamines; therapeutic use; Hypoxia; physiopathology; Male; Propanolamines; therapeutic use; Pulmonary Alveoli; drug effects; metabolism; pathology; Pulmonary Edema; drug therapy; etiology; metabolism; Rats; Rats, Wistar
- From: Chinese Medical Journal 2010;123(8):1028-1033
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDRecent research suggests that beta(2)-adrenergic agonists increase alveolar fluid clearance (AFC) under physiologic and pathologic conditions. It is unknown whether beta(3)-adrenergic agonists also increase AFC under pathologic conditions. The aim of this study was to investigate the effect of beta(3)-adrenergic agonists on AFC following hypoxic lung injury and the mechanisms involved.
METHODSHypoxic rats were exposed to 10% oxygen. BRL-37344 (beta(3)-adrenergic agonist) or CGP-12177 (selective beta(3)-adrenergic agonist) alone or combined with beta receptor antagonists, sodium channel blockers, or Na(+)/K(+)-ATPase blockers were perfused into the alveolar space of rats exposed to 10% oxygen for 48 hours. Total lung water content (TLW) and AFC were measured.
RESULTSAFC did not change for the first 24 hours but then decreased after 48-hour exposure to 10% oxygen. The perfusion of BRL-37344 or CGP-12177 significantly increased AFC in normal and hypoxic rats. The AFC-stimulating effect of CGP-12177 was lowered with amiloride (a Na(+) channel blocker) and ouabain (a Na(+)/K(+)-ATPase inhibitor) by 37% and 49%, respectively. Colchicine significantly inhibited the effect of CGP-12177.
CONCLUSIONSThese findings suggest that beta(3)-adrenergic agonists can increase AFC during hypoxic lung injury in rats and accelerate the amelioration of pulmonary edema.
