Synthesis of diisooctyl adipate catalyzed by lipase-displaying Pichia pastoris whole-cell biocatalysts.
- Author:
Na ZHANG
1
;
Zi JIN
;
Ying LIN
;
Suiping ZHENG
;
Shuangyan HAN
Author Information
1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China.
- Publication Type:Journal Article
- MeSH:
Adipates;
metabolism;
Industrial Microbiology;
Lipase;
metabolism;
Pichia;
metabolism
- From:
Chinese Journal of Biotechnology
2013;29(7):1027-1031
- CountryChina
- Language:Chinese
-
Abstract:
An enzyme-displaying yeast as a whole-cell biocatalyst is an alternative to immobilized enzyme, due to its low-cost preparation and simple recycle course. Here, lipase-displaying Pichia pastoris whole-cell was used as a biocatalyst to synthesize diisooctyl adipate in the non-aqueous system. The maximum productivity of diisooctyl adipate was obtained as 85.0% in a 10 mL reaction system. The yield could be reached as high as 97.8% when the reaction system was scaled up to 200 mL. The purity obtained is 98.2% after vacuum distillation. Thus, the lipase-displaying P. pastoris whole-cell biocatalyst was promising in commercial application for diisooctyl adipate synthesis in non-aqueous phase.
