Construction and fermentation control of reductive TCA pathway for malic acid production in Saccharomyces cerevisiae.
- Author:
Daojiang YAN
1
;
Caixia WANG
1
;
Jiemin ZHOU
1
;
Yilan LIU
1
;
Maohua YANG
1
;
Jianmin XING
1
Author Information
1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
- Publication Type:Journal Article
- MeSH:
Citric Acid Cycle;
Fermentation;
Industrial Microbiology;
methods;
Malate Dehydrogenase;
genetics;
metabolism;
Malates;
metabolism;
Metabolic Engineering;
methods;
Metabolic Networks and Pathways;
Oxidation-Reduction;
Pyruvate Carboxylase;
genetics;
metabolism;
Saccharomyces cerevisiae;
genetics;
metabolism;
Signal Transduction
- From:
Chinese Journal of Biotechnology
2013;29(10):1484-1493
- CountryChina
- Language:Chinese
-
Abstract:
Malic acid is widely used in food, and chemical industries. Through overexpressing pyruvate carboxylase and malate dehydrogenase in pdc1-deficient Saccharomyces cerevisiae, malic acid was successfully produced through the reductive TCA pathway. No malic acid was detected in wild type Saccharomyces cerevisiae, however, 45 mmol/L malic acid was produced in engineered strain, and the concentration of byproduct ethanol also reduced by 18%. The production of malic acid enhanced 6% by increasing the concentration of Ca2+. In addition, the final concentration reached 52.5 mmol/L malic acid by addition of biotin. The increasing is almost 16% higher than that of the original strain.
