A method to determine the structure of the complex of enzyme and its substrate using 6-phosphate-beta-glucosidase at low temperature.
- Author:
Wei WANG
;
Yiwei LIU
;
Hongbin LI
- Publication Type:Journal Article
- MeSH:
Cold Temperature;
Crystallization;
Glucosidases;
chemistry;
metabolism;
Substrate Specificity;
X-Ray Diffraction
- From:
Chinese Journal of Biotechnology
2013;29(12):1828-1835
- CountryChina
- Language:Chinese
-
Abstract:
To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C, we soaked the crystal of 6-phosphate-beta-glucosidase (Bg1A) in the liquor containing p-nitrophenyl-beta-D-glucopyranoside-6-phosphate (pNPbetaG6P). The diffraction data at 2 A resolution was collected and an intact and unambiguous electron density map of pNPbetaG6P was obtained. These results provide an effective method for the research of cryoenzymology and the intermediate state of enzyme-substrate complex in the future.
