Comparison of three approaches to breed industrial Saccharomyces cerevisiae strains with improved ethanol tolerance.
- Author:
Qian LI
;
Xinqing ZHAO
;
Jin-Soo KIM
;
Fengwu BAI
- Publication Type:Journal Article
- MeSH:
Adaptation, Physiological;
drug effects;
Drug Resistance, Fungal;
genetics;
Ethanol;
pharmacology;
Fungal Proteins;
genetics;
metabolism;
Industrial Microbiology;
methods;
Mutagenesis;
Saccharomyces cerevisiae;
drug effects;
genetics;
growth & development
- From:
Chinese Journal of Biotechnology
2013;29(11):1672-1675
- CountryChina
- Language:Chinese
-
Abstract:
Ethanol tolerance is related to the expression of multiple genes, and genome-based engineering approaches are much more efficient than manipulation of single genes. In this study, ultraviolet (UV) mutagenesis, dielectric barrier discharge (DBD) air plasma mutagenesis, and artificial transcription factor (ATF) technology were adopted to treat an industrial yeast strain S. cerevisiae Sc4126 to obtain mutants with improved ethanol tolerance. Mutants with high ethanol tolerance were obtained, and the ratio of positive mutants was compared. Among the three approaches, the rate of positive mutation obtained by ATF technology was 10- to 100-folds of that of the two other methods, with highest genetic stability, suggesting the ATF technology promising for rapid alteration of phenotypes of industry yeast strains for efficient ethanol fermentation.
