Relationship between mycelium morphology and laccase production of Pleurotus ferulae in submerged cultivation.
- Author:
Youzhi CHEN
;
Lu WANG
;
Lin PENG
;
Zhongyang DING
;
Liang ZHANG
;
Zhenghua GU
;
Guiyang SHI
;
Kechang ZHANG
- Publication Type:Journal Article
- MeSH:
Culture Media;
Fermentation;
Glass;
chemistry;
Industrial Microbiology;
Laccase;
biosynthesis;
Mycelium;
cytology;
growth & development;
Pleurotus;
cytology;
enzymology
- From:
Chinese Journal of Biotechnology
2013;29(11):1701-1705
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the relationship between mycelium morphology and laccase production was studied. The results indicated that the morphology of P. ferulae pellets was changed when glass beads were added. Laccase production showed higher with spherical mycelium than with filamentous or flocculent mycelium. In addition, the spherical mycelium with a diameter of 0.2-0.4 mm highly affected laccase production. Effect of the composition of culture medium on pellets was investigated and results indicated that various concentrations of glucose, corn meal and wheat bran were important to the formation of pellets in diameter of 0.2-0.4 mm. Besides nutrients, the addition of non-nutritional substrates influenced the distribution of P. ferulae pellets. However, the production of laccase was not promoted by non-nutritional substrates.