Improved extraction of solanesol from tobacco waste by enzymatic cell wall breaking.
- Author:
Xingmin WANG
;
Yuwen ZHANG
;
Guizhi ZHANG
;
Zhongyi YIN
- Publication Type:Journal Article
- MeSH:
Cell Wall;
metabolism;
Cellulase;
metabolism;
Oxygenases;
metabolism;
Plant Leaves;
chemistry;
Terpenes;
isolation & purification;
Tobacco;
chemistry
- From:
Chinese Journal of Biotechnology
2013;29(11):1706-1710
- CountryChina
- Language:Chinese
-
Abstract:
To improve the extraction of solanesol from tobacco waste, we developed an enzymatic cell wall-breaking process with combined cellulase and ligninase. The effects of reaction time, temperature, pH and enzyme/substrate ratio were determined. The results show that the catalytic effect was better than either single enzyme when the ratio of cellulase to ligninase was 15:1 (U/U). Under the optimized conditions of 175 U/g (enzymes/substrate), tobacco to water 1:5 (W/W), temperature 40 degrees C and pH 6.0, the concentration of solanesol in the solution could reach 0.33 g/L after 8 h. And the average leaching rate reached 96.53% which was 1.68 times of the extraction methods of chemical reflux. It provides new way for the extraction of solanesol from tobacco waste, and worthwhile to be further explored.
