Production of sugar syrup containing rare sugar using dual-enzyme coupled reaction system.
- Author:
Wenjia HAN
;
Yueming ZHU
;
Wei BAI
;
Ken IZUMORI
;
Tongcun ZHANG
;
Yuanxia SUN
- Publication Type:Journal Article
- MeSH:
Aldose-Ketose Isomerases;
metabolism;
Carbohydrate Epimerases;
metabolism;
Fructose;
chemistry;
Glucose;
chemistry;
Hydrogen-Ion Concentration;
Temperature
- From:
Chinese Journal of Biotechnology
2014;30(1):90-97
- CountryChina
- Language:Chinese
-
Abstract:
Enzymatic conversion is very important to produce functional rare sugars, but the conversion rate of single enzymes is generally low. To increase the conversion rate, a dual-enzyme coupled reaction system was developed. Dual-enzyme coupled reaction system was constructed using D-psicose-3-epimerase (DPE) and L-rhamnose isomerase (L-RhI), and used to convert D-fructose to D-psicose and D-allose. The ratio of DPE and L-RhI was 1:10 (W/W), and the concentration of DPE was 0.05 mg/mL. The optimum temperature was 60 degrees C and pH was 9.0. When the concentration of D-fructose was 2%, the reaction reached its equilibrium after 10 h, and the yield of D-psicose and D-allose was 5.12 and 2.04 g/L, respectively. Using the dual-enzymes coupled system developed in the current study, we could obtain sugar syrup containing functional rare sugar from fructose-rich raw material, such as high fructose corn syrup.
