Effect of ginseng-sanqi extract on the Ras associated signal proteins.
- Author:
Wei TIAN
1
;
Yan LEI
;
Ling-Qun ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Ginsenosides; pharmacology; Human Umbilical Vein Endothelial Cells; drug effects; metabolism; Humans; Mitogen-Activated Protein Kinases; metabolism; Signal Transduction; Vascular Endothelial Growth Factor Receptor-2; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2009;29(9):802-805
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of ginseng-sanqi extract (GSE) on the vascular endothelium growth factor receptor-2 (VEGFR-2), Ras and mitogen-activated protein kinase (MAPK) in VEGFR-2-Ras-MAPK signal pathway of angiogenesis in cultured human umbilical vascular endothelial cells (HUVECs).
METHODSThe protein expressions of VEGFR-2, Ras and MAPK in HUVEC and the effects of GSE (at different doses) and basic fibrin growth factor (bFGF) on them were detected by Western-Blot test.
RESULTSGSE can enhance the expression of angiogenesis signaling proteins (VEGFR-2, Ras, MAPK) to different extents, especially in the groups treated by bFGF or higher dose GSE, the levels showed significant differences to those in the blank group (P<0.05).
CONCLUSIONGSE can promote the expressions of VEGFR-2, Ras and MAPK in the angiogenesis signaling pathway, which may be the foundation of Chinese medicine for tonifying qi and activating blood circulation in promoting endothelial proliferation and angiogenesis.
