Effect of cinobufagin on the expressions of bcl-2 mRNA and bax mRNA and the proliferation of lens epithelial cells.
- Author:
Ting-Ting WANG
1
;
Guo-Xing XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Bufanolides; pharmacology; Cell Proliferation; drug effects; Epithelial Cells; drug effects; metabolism; Lens, Crystalline; cytology; Proto-Oncogene Proteins c-bcl-2; metabolism; RNA, Messenger; genetics; Rabbits; bcl-2-Associated X Protein; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2009;29(10):915-917
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of cinobufagin on the proliferation of lens epithelial cells (LECs) and the bcl-2 and bax mRNA expressions of rabbits.
METHODSCultured LECs were treated for 72 h with cinobufagin of different concentrations, the end titer was 0.1 mg/L for low, 0.2 mg/L for moderate and 0.3 mg/L for high concentration, respectively. The inhibitory rate of cinobufagin on LECs' proliferation was detected using MTT method; the mRNA expressions of bcl-2 and bax genes in LECs were examined using reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSProliferation of LECs was inhibited significantly by cinobufagin in a concentration dependent manner, the inhibitory rate was 24.65%, 30.13% and 36.98% respectively for low, moderate and high concentration. With the drug concentration increasing, the mRNA expression levels of the pro-apoptotic bax were increased, whereas those of the anti-apoptotic bcl-2 decreased.
CONCLUSIONCinobufagin can remarkably inhibit the proliferation and induce the apoptosis of LECs in vitro, it might be taken as one of the drugs for the prevention and treatment of after-cataract.
