Effect of ginsenosides on level of sex hormone receptors in human liver cell line HL-7702.
- Author:
Yong LI
1
;
Da-li ZHANG
;
Peng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Ginsenosides; pharmacology; Hepatocytes; cytology; drug effects; metabolism; Humans; Receptors, Estrogen; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2009;29(12):1110-1113
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of ginsenoside (GSS) in regulating level of sex hormone receptors in human liver cell line HL-7702.
METHODSThe growth of HL-7702 were detected by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay for choosing the available concentration of GSS; and the effect of GSS on sex hormone receptors in HL-7702 cells were detected by immuno-histochemistry, Western blot and RT-PCR.
RESULTSGSS significantly enhance the protein and mRNA expressions of estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR) in HL7702 cell in a dose-dependent manner, the levels of expressions in the GSS treated group were higher than those in the solvent control group respectively (P < 0.05).
CONCLUSIONGSS can up-regulate the protein and mRNA expressions of sex hormone receptors in HL-7702.
