Effect of human follicular fluid from endometriosis patients treated with quyu jiedu granule on mouse embryonic development.
- Author:
Xin-ling LI
1
;
Fang LIAN
;
Yan-he LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blastocyst; Drugs, Chinese Herbal; therapeutic use; Embryo, Mammalian; drug effects; Embryonic Development; drug effects; Endometriosis; drug therapy; Female; Follicular Fluid; Humans; Mice; Mice, Inbred Strains; Phytotherapy
- From: Chinese Journal of Integrated Traditional and Western Medicine 2009;29(11):1001-1004
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of human follicular fluid (HFF) from endometriosis (EM) patients treated with Quyu Jiedu Granule (QJG) on mouse embryonic development.
METHODSCultured 2-cell mouse embryos were divided into three groups. To the medium of Group A (70 embryos), HFF from endometriosis patients treated with QJG was added; to that of Group B (60 embryos), HFF from endometriosis patients untreated with QJG, and to Group C (59 embryos), HFF from patients with fallopian tube obstruction was added. The percentages of embryos developed to 8-cell stage, morula stage and blastula stage were counted, and the early stage number and rate of high-quality embryo were measured.
RESULTSAmong the 70 embryos in Group A, 53 (75.71%) developed to 8-cell stage, 48 (68.57%) to morula stage and 45 (64.28%) to blastula stage; while in the 60 embryos of Group B, the corresponding number (percentage) were 34 (56.67%), 29 (48.33%), 21 (35.00%), respectively, showing significant differences between groups (P < 0.05). High-quality of embryo was 61 (87.14%) in Group A and 44 (73.3%) in Group B, the difference between groups also showed statistical significance (P < 0.05).
CONCLUSIONHFF from endometriosis patients is toxic to 2-cell mouse embryos, but after treated by QJG, it could elevate the quality of cultured mouse embryo in the early stage.
