Effect of er'zhi tiangui granule on metabonomics and level of Ca2+ in follicle fluid in patients after in vitro fertilization and embryo transfer.
- Author:
Fang LIAN
1
;
Bin ZHAO
;
Xue-mei LU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Calcium; metabolism; Drugs, Chinese Herbal; pharmacology; therapeutic use; Embryo Transfer; Female; Fertilization in Vitro; Follicular Fluid; drug effects; metabolism; Humans; Infertility, Female; etiology; metabolism; Metabolomics; Oocytes; metabolism; Phytotherapy; Pregnancy
- From: Chinese Journal of Integrated Traditional and Western Medicine 2010;30(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of action of Er'zhi Tiangui Granule (ETG) in improving quality of oocytes by observing metabonomics and level of calcium ion in follicle fluid, and to investigate the impacts of calcium ion, cholinesterase (ChE) and creatinine (CCr) levels in human follicle fluid on the quality of oocytes and outcome of pregnancy in patients after in vitro fertilization and embryo transfer (IVF-ET).
METHODSFifty-seven patients after IVF-ET were randomly assigned to two groups: the trial group (27 patients) and the control group (30 patients), both were treated with conventional Western medical treatment, but ETG and Liuwei Dihuang Granule (LDG) was given respectively to the two groups additionally. Changes of Shen-asthenia syndrome, amount of oocyte obtained, fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate, levels of calcium ion, ChE and Cr in follicle fluid, and metabonomics in the two groups were observed and compared.
RESULTSAfter treatment, scores of Shen-asthenia syndrome 8.30 +/- 1.46, fertilization rate 0.82 +/- 0.09, cleavage rate 0.97 +/- 0.07 and high-quality embryos rate 0.51 +/- 0.18 in the trial group were all better than those in the control group (9.16 +/- 1.15, 0.74 +/- 0.18, 0.91 +/- 0.10, 0.41 +/- 0.09, respectively, P<0.05); metabonomics principal component analysis showed that in the trial group, the principal component in follicle fluid distributed mainly in the section I, and that in the control group distributed mainly in the section II, showing significant difference between the two groups; while in most pregnant patients, it distributed in the section I. The Ca2+ concentration in the trial group was significantly higher than that in the control group (P<0.05). Levels of ChE and Cr in the trial group were higher than those in the control group, but the difference between them was insignificant.
CONCLUSIONETG is better than LDG in regulating metabonomics, Ca2+ concentration, improving the quality of oocyte and embryo, and increasing pregnant rate in patients after IVF-ET.
