Protective effect and mechanism of β-CM7 on renin angiotensin system & diabetic cardiomyopathy.
- Author:
Kun WANG
;
Dongning HAN
;
Yujuan ZHANG
;
Chao RONG
;
Yuanshu ZHANG
- Publication Type:Journal Article
- MeSH:
Angiotensin II;
metabolism;
Animals;
Diabetes Mellitus, Experimental;
drug therapy;
Diabetic Cardiomyopathies;
drug therapy;
Endorphins;
pharmacology;
Male;
Myocardium;
metabolism;
pathology;
Peptide Fragments;
pharmacology;
Peptidyl-Dipeptidase A;
metabolism;
RNA, Messenger;
Rats;
Rats, Sprague-Dawley;
Receptor, Angiotensin, Type 1;
metabolism;
Receptors, G-Protein-Coupled;
metabolism;
Renin-Angiotensin System
- From:
Chinese Journal of Biotechnology
2016;32(2):195-203
- CountryChina
- Language:Chinese
-
Abstract:
This article aimed at exploring the effects and protective mechanism of β-CM7 on renin angiotensin system (RAS) in diabetic rats myocardial tissue. We divided 32 male SD rats into 4 groups: control group, diabetic model control group, insulin (3.7x10(-8) mol/d) treatment group and β-CM7 (7.5x10(-8) mol/d) treatment group. After 30 days, all rats were decapitated and myocardical tissues were collected immediately. After injection, β-CM7 could decrease the content of Ang II, increase the content of Angl-7. And β-CM7 could improve the mRNA of AT1 receptor and Mas receptor. β-CM7 also could improve the mRNA of ACE and ACE2, enhance the activity of ACE and ACE2. These data confirmed tli β-CM7 could activate ACE2-Angl-7-Mas axis, negative passage in RAS, to inhibit the expression ACE mnRiJA and protein in rat myocardium, alleviate the myocardial tissue damage induced by Ang II. The effect of β-CM7 on inhibiting myocardium damage might be related to ACE/ACE2 passageway.
