Role of NO signal in ABA-induced phenolic acids accumulation in Salvia miltiorrhiza hairy roots.
- Author:
Lihong SHEN
;
Jiahui REN
;
Wenfang JIN
;
Ruijie WANG
;
Chunhong NI
;
Mengjiao TONG
;
Zongsuo LIANG
;
Dongfeng YANG
- Publication Type:Journal Article
- MeSH:
Abscisic Acid;
pharmacology;
Benzofurans;
metabolism;
Free Radical Scavengers;
pharmacology;
Hydroxybenzoates;
metabolism;
Nitric Oxide;
metabolism;
Phenylalanine Ammonia-Lyase;
metabolism;
Plant Roots;
metabolism;
Salvia miltiorrhiza;
metabolism;
Tyrosine Transaminase;
metabolism
- From:
Chinese Journal of Biotechnology
2016;32(2):222-230
- CountryChina
- Language:Chinese
-
Abstract:
To investigate roles of nitric oxide (NO) signal in accumulations of phenolic acids in abscisic.acid (ABA)-induced Salvia miltiorrhiza hairy roots, S. miltiorrhiza hairy roots were treated with different concentrations of sodium nitroprusside (SNP)-an exogenous NO donor, for 6 days, and contents of phenolic acids in the hairy roots are determined. Then with treatment of ABA and NO scavenger (2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1- oxyl-3-oxide, c-PTIO) or NO synthase inhibitor (NG-nitro-L-arginine methyl ester, L-NAME), contents of phenolic acids and expression levels of three key genes involved in phenolic acids biosynthesis were detected. Phenolic acids production in S. miltiorrhiza hairy roots was most significantly improved by 100 µmoL/L SNP. Contents of RA and salvianolic acid B increased by 3 and 4 folds. ABA significantly improved transcript levels of PAL (phenylalanine ammonia lyase), TAT (tyrosine aminotransferase) and RAS (rosmarinic acid synthase), and increased phenolic acids accumulations. However, with treatments of ABA+c-PTIO or ABA+L-NAME, accumulations of phenolic acids and expression levels of the three key genes were significantly inhibited. Both NO and ABA can increase accumulations of phenolic acids in S. miltiorrhiza hairy roots. NO signal probably mediates the ABA-induced phenolic acids production.
