The modified bacterial two-hybrid system.
- Author:
Junyang WANG
;
Weishan WANG
;
Hua ZHAO
;
Keqian YANG
- Publication Type:Journal Article
- MeSH:
Bacteria;
Genes, Reporter;
Promoter Regions, Genetic;
Protein Interaction Mapping;
methods;
Two-Hybrid System Techniques
- From:
Chinese Journal of Biotechnology
2016;32(2):231-240
- CountryChina
- Language:Chinese
-
Abstract:
Bacterial two-hybrid system is a newly developed method for studying protein-protein interactions. However, in our studies of the interaction of regulatory proteins in Streptomyces, it was found that the bacterial two-hybrid system is not sensitive enough by the blue-and-white selection on X-gal plate. To overcome this drawback, the reason of false positive clone was firstly determined, which was the disturbance of other direct or indirect regulation on lacZ promoter. Then the disturbance was diluted by introducing multicopy lacZ promoter, which drive another reporter gene gfp. By such design, the sensitivity of the modified bacterial two-hybrid system was significantly inproved and the two different reporters also help to decrease the rate of the false positive clones. Further the evaluation of the modifiedd bacterial two-hybrid system indicated that the sensitivity was significantly improved.
