Effect of integration loci of genome on heterologous gene expression in Saccharomyces cerevisiae.

Author: Wenzheng ZHANG ¹ ; Jijun TANG ² ; Bingzhi LI ¹ ; Yingjin YUAN ¹
Author Information

1. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China.
2. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Publication Type:Journal Article
Keywords: Saccharomyces cerevisiae; chromosomal region; heterologous gene expression; synthetic biology
MeSH: Gene Expression Regulation, Fungal; Gene Knock-In Techniques; Genes, Reporter; Genome, Fungal; Saccharomyces cerevisiae; genetics
From: Chinese Journal of Biotechnology 2016;32(7):901-911
CountryChina
Language:Chinese
Abstract: Chromosomal integration of heterologous genes or pathways is preferred over the use of episomal plasmids for its inherently stability and thus more desirable in the industrial setting. However, the position of integration of heterologous genes in the genome influences the expression levels. In combination of high throughput transformation of the Yeast Knock-out Collection (YKO) and FACS analysis, the position effect on heterologous reporter gene gfp was identified across the whole genome in yeast. In total 428 high-expressed sites and 444 low-expressed sites were spotted, providing massive data to analyze patterns and reasons for region dependency of gene expression on the genome-wide scale.