An XcmⅠ-generated T vector and its applications in synthetic biology.

Author: Yangkai DUAN ¹ ; Feiyan LIANG ¹ ; Xiaoming TAN ² ; Xuefeng LV ²
Author Information

1. University of Chinese Academy of Sciences, Beijing 100049, China.
2. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China.
Publication Type:Journal Article
Keywords: Biobrick standard; TA cloning; synthetic biology
MeSH: Cloning, Molecular; DNA; Genetic Vectors; Plasmids; Polymerase Chain Reaction; Synthetic Biology
From: Chinese Journal of Biotechnology 2016;32(7):956-965
CountryChina
Language:Chinese
Abstract: For more economical and efficient DNA clonging, pFL-XS-T, a Biobrick-T vector was constructed based on pMD18-T vector, carrying clonging regions of XbaⅠ-XcmⅠ-XcmⅠ-SpeⅠ. The results revealed that PCR products could be conveniently inserted into pFL-XS-T vevtor digested by XcmⅠby means of TA cloning. The positive frequency of recombination can meet the experimental requirements and all the plasmids obtained meet Biobrick standard. Moreover, the pFL-XS-T is compatible with other Biobrick parts, and serves as a vector for functional DNA fragments screening.