In vitro development and chimeric efficiency of mouse-porcine interspecies chimeric embryos in different culture systems.

Ying Wang; Jilong Ren; Yuran Song; Tang Hai; Qi Zhou; Zhonghua Liu

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In vitro development and chimeric efficiency of mouse-porcine interspecies chimeric embryos in different culture systems.

Author: Ying WANG ¹ ; Jilong REN ² ; Yuran SONG ² ; Tang HAI ² ; Qi ZHOU ² ; Zhonghua LIU ¹
Author Information

1. College of Life Science, Northeast Agricultural University of China, Harbin 150030, Heilongjiang, China.
2. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Publication Type:Journal Article
Keywords: chimeric injection; embryo development in vitro; induced pluripotent stem cells; interspecies chimera; porcine
MeSH: Animals; Blastocyst; Chimera; Culture Media; Embryo Culture Techniques; veterinary; Embryo, Mammalian; Embryonic Development; Induced Pluripotent Stem Cells; cytology; Mice; Swine
From: Chinese Journal of Biotechnology 2016;32(7):975-985
CountryChina
Language:Chinese
Abstract: With the advancements of stem cells and regenerative medicine, interspecies chimera has become a hot topic and will pave a new way of providing donor sources in organ transplantation. However, the interspecies chimera is confronted with a number of scientific questions and technical obstacles, including selections of appropriate embryonic stage and appropriate culture medium; those factors will deeply influence the developmental balance between donor cells and receptor embryos. Due to its relatively rapid reproductive cycle and similar organ size to human's, porcine is a very potential donor candidate to study these questions. To compare the development and chimeric efficiency of interspecies embryos, we tested and evaluated three different culture systems, PZM-3 (Porcine zygotic medium), culture medium for iPSCs (N2B27) and 3.5 h of N2B27 before PZM-3 (N2B27(3.5 h)), and two different embryonic stages, 8-cell and blastocyst in mouse-porcine chimeric embryos using parthenogenetically activated porcine embryos and mouse induced pluripotent stem cells (miPS). The results showed that, PZM-3 was beneficial for both development of chimeric embryos and miPSCs proliferation in porcine embryos in the 8-cell injection group. After early blastocyst injection, the chimeric efficiency did not appear significantly different among the three culture systems but was lower than 8-cell injection. In summary, the results suggest that 8-cell injection and PZM-3 culture medium are more beneficial to the in vitro development and chimeric efficiency of mouse-porcine chimeric embryos.