N-terminomics: proteomic strategies for protein N-terminal profiling.

Zhiqiang Wang; Yao Zhang; Xuechuan Hong; Ping XU

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N-terminomics: proteomic strategies for protein N-terminal profiling.

Author: Zhiqiang WANG ¹ ; Yao ZHANG ² ; Xuechuan HONG ¹ ; Ping XU ¹
Author Information

1. Key Laboratory of Combinational Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Science, Wuhan University, Wuhan 430072, Hubei, China.
2. National Engineering Research Center for Protein Drugs, Beijing Proteome Research Center, National Center for Protein Sciences, State Key Laboratory of Proteomics, Beijing Institute of Radiation Medicine, Beijing 102206, China.
Publication Type:Journal Article
Keywords: enrichment; mass spectrometry; protein N-termini; proteomics
MeSH: Mass Spectrometry; Protein Processing, Post-Translational; Proteins; chemistry; Proteomics
From: Chinese Journal of Biotechnology 2016;32(8):1001-1009
CountryChina
Language:Chinese
Abstract: Protein N-termini, as the beginning of translation, has a major impact on protein's biological functions. Its sequence and various post-translational modifications often affect protein activation, stability and cellular-localization, regulate the signal transduction, and even determine protein's final destiny. The systematic study of protein N-termini can clarify the vital function of the N-terminus, and provide in-depth knowledge of the multifunctional roles that protein has played in diverse biological processes. In addition, N-terminal research may help us to achieve high-coverage proteomics and re-annotate genomics. Combined with our own research, this review highlights recent progress of N-terminomics, especially some important enrichment strategies and technologies based on mass spectrometry.