Advances and prospects of taxol biosynthesis by endophytic fungi.

Kai Zhao; Lu YU; Yuyan Jin; Xueling MA; Dan Liu; Xiaohua Wang; Xin Wang

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Advances and prospects of taxol biosynthesis by endophytic fungi.

Author: Kai ZHAO ¹ ; Lu YU ¹ ; Yuyan JIN ¹ ; Xueling MA ² ; Dan LIU ¹ ; Xiaohua WANG ¹ ; Xin WANG ¹
Author Information

1. Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, Heilongjiang, China.
2. Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
Publication Type:Journal Article
Keywords: biosynthesis; endophytic fungi; related genes; taxol
MeSH: Endophytes; Fungi; Industrial Microbiology; Microorganisms, Genetically-Modified; Neoplasms; drug therapy; Paclitaxel; biosynthesis; Taxus; chemistry
From: Chinese Journal of Biotechnology 2016;32(8):1038-1051
CountryChina
Language:Chinese
Abstract: Taxol is one of the most important chemotherapeutic drugs against cancer. Taxol has been mainly extracted from the bark of yews for a long time. However, methods for the extraction of taxol from the bark of Taxus species were inefficient and environmentally costly. As a result of the high ecological toll exacted on trees with the potential for Pacific yew extinction, investigators began to look for other methods of taxol production. Recently, increasing efforts have been made to develop alternative means of taxol production, such as using complete chemical synthesis, semi-synthesis, Taxus spp. plant cell culture and microbe fermentation. Using microbe fermentation in the production of taxol would be a very prospective method for obtaining a large amount of taxol. Therefore, it is necessary to understand the molecular basis and genetic regulation mechanisms of taxol biosynthesis by endophytic fungi, which may be helpful to construct the genetic engineering strain with high taxol output. In this paper, the taxol biosynthesis pathway from Taxus cells and the advantages of taxol biosynthesis by endophytic fungi were discussed. The study on the isolation and biodiversity of taxol-producing endophytic fungi and the taxol biosynthesis related genes are also discussed.