Hyaluronic acid production by Streptococcus iniae and its application in rabbit skin's regeneration.

Feng Guan; Jiachang Jin; Huaguo Zhao; Lei Hong; Zhisen Shen; Yabin Zhu

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Hyaluronic acid production by Streptococcus iniae and its application in rabbit skin's regeneration.

Author: Feng GUAN ¹ ; Jiachang JIN ¹ ; Huaguo ZHAO ¹ ; Lei HONG ¹ ; Zhisen SHEN ² ; Yabin ZHU ¹
Author Information

1. The Medical School, Ningbo University, Ningbo 315211, Zhejiang, China.
2. The Lihuili Hospital Affiliated to the Medical School, Ningbo University, Ningbo 315041, Zhejiang, China.
Publication Type:Journal Article
Keywords: Streptococcus iniae; fermentation; hyaluronic acid; skin regeneration
MeSH: Animals; Biocompatible Materials; Fermentation; Hyaluronic Acid; biosynthesis; Hydrogels; Molecular Weight; Rabbits; Regeneration; Skin; drug effects; growth & development; Streptococcus iniae; metabolism; Wound Healing
From: Chinese Journal of Biotechnology 2016;32(8):1104-1114
CountryChina
Language:Chinese
Abstract: Hyaluronic acid (HA) is an important biomaterial as the extracellular matrix in human body. We produced HA by fermentation of Streptococcus iniae (Strep.). Production of HA by Strep. was evaluated and further improved by strain mutation by ultraviolet. One strain with higher HA yield and lower content of protein was obtained. Its HA yield increased from (82.3±3.3) mg/L to (120±10.6) mg/L, and protein decreased from (0.178±0.011) mg/L to (0.032±0.017) mg/L. The molecular weight (MW) of HA yield from Strep. is about 3.0×10⁵ Da. Using the method of freezing and thawing, HA aqueous solution was transferred into hydrogel. This HA hydrogel, casted on sterilized non-woven fabric, was applied to repair rabbit skin with full-thickness defect. The preliminary results of the animal tests displayed that HA hydrogel obviously reduced the inflammation around the wound and promoted the skin regeneration comparing with the control tests.